Journal: CNS Neuroscience & Therapeutics

Article Title: Acetyl‐11‐keto‐beta‐boswellic acid modulates macrophage polarization and Schwann cell migration to accelerate spinal cord injury repair in rats

doi: 10.1111/cns.14642

Figure Lengend Snippet: Information of primary antibodies.

Article Snippet: NRF2 , 1:1000 , Rabbit , bs‐1074R , AB_10855421 , Bioss (Beijing, China).

Techniques: Concentration Assay

Journal: Biomedicines

Article Title: Cadmium-Induced Kidney Injury in Mice Is Counteracted by a Flavonoid-Rich Extract of Bergamot Juice, Alone or in Association with Curcumin and Resveratrol, via the Enhancement of Different Defense Mechanisms

doi: 10.3390/biomedicines9121797

Figure Lengend Snippet: Oligonucleotide primers used for the quantitative Real-time PCR analyses.

Article Snippet: Primary antibodies IL-1β (1:250, Santa Cruz Biotechnology, Dallas, TX, USA) and Nrf2 (1:150, St. John’s Laboratory, London, UK) were incubated overnight at 4 °C in a moisturized chamber.

Techniques: Real-time Polymerase Chain Reaction, Sequencing

Journal: Biomedicines

Article Title: Cadmium-Induced Kidney Injury in Mice Is Counteracted by a Flavonoid-Rich Extract of Bergamot Juice, Alone or in Association with Curcumin and Resveratrol, via the Enhancement of Different Defense Mechanisms

doi: 10.3390/biomedicines9121797

Figure Lengend Snippet: Real-time PCR analysis of Nrf2 ( A ), Nqo1 ( B ) and Hmox1 ( C ). *** p < 0.001 vs. control mice; §§§ p < 0.001 vs. CdCl 2 -treated mice.

Article Snippet: Primary antibodies IL-1β (1:250, Santa Cruz Biotechnology, Dallas, TX, USA) and Nrf2 (1:150, St. John’s Laboratory, London, UK) were incubated overnight at 4 °C in a moisturized chamber.

Techniques: Real-time Polymerase Chain Reaction, Control

Journal: Biomedicines

Article Title: Cadmium-Induced Kidney Injury in Mice Is Counteracted by a Flavonoid-Rich Extract of Bergamot Juice, Alone or in Association with Curcumin and Resveratrol, via the Enhancement of Different Defense Mechanisms

doi: 10.3390/biomedicines9121797

Figure Lengend Snippet: Immunohistochemical localization of Nrf2 in the kidneys. ( A ) In all control groups, Nrf2 immunoreactivity is particularly strong in the tubular wall (arrow). ( B ) In CdCl 2 plus vehicle-treated mice, no Nrf2 immunoreactivity is present. ( C – E ) In mice treated with CdCl 2 plus both doses of Cur and with CdCl 2 plus Re, Nrf2 shows a moderate positivity in some tubules (arrow). ( F ) In mice challenged with CdCl 2 plus BJe at the lower dose, Nrf2 immunoreactivity was lower (arrow) when compared to Cur and Re. ( G – I ) Mice treated with CdCl 2 plus BJe at 40 mg/kg and with CdCl 2 plus both associations: Nrf2 immunoreactivity is high (arrow), similar to controls. ( J ) Morphometric results for Nrf2 expression. Data are expressed in optical units/unit area (OU/UA) (from 0 = black to 255 = white). * p < 0.05 vs. control; § p < 0.05 vs. CdCl 2 plus vehicle. Scale bar: 50 µm.

Article Snippet: Primary antibodies IL-1β (1:250, Santa Cruz Biotechnology, Dallas, TX, USA) and Nrf2 (1:150, St. John’s Laboratory, London, UK) were incubated overnight at 4 °C in a moisturized chamber.

Techniques: Immunohistochemical staining, Control, Expressing

Journal: Neural Regeneration Research

Article Title: Electroacupuncture alleviates cerebral ischemia and reperfusion injury via modulation of the ERK1/2 signaling pathway

doi: 10.4103/1673-5374.187041

Figure Lengend Snippet: Effects of EA on mRNA expression levels of Bax, Bcl-2, GCS and Nrf2 in the ischemic cortex after MCAO. Bax (A), Bcl-2 (B), GCSh (D), GCSl (E) and Nrf2 (F) mRNA expression levels, as assessed by real-time PCR. The Bax/Bcl-2 ratio is shown in C. Data ( n = 7 per group in A–C, n = 5 per group in D–F) are expressed as the mean ± SEM. Statistical significance was determined using analysis of variance followed by Tukey’s multiple comparison test for multiple comparisons. * P < 0.05, ** P < 0.01, vs . sham (sham-operation) group; # P < 0.05, vs . MCAO group; † P < 0.05, vs. EA (MCAO + EA) group. MCAO: Middle cerebral artery occlusion; EA: electroacupuncture; EA plus PD98059: MCAO + EA + PD98059; GCSh: gamma-glutamylcysteine synthetase heavy subunit; GCSl: gamma-glutamylcysteine synthetase light subunit; Nrf2: nuclear factor erythroid 2-related factor 2.

Article Snippet: Then, the sections were incubated with rabbit anti-rat GCS monoclonal antibody (1:100; BA1627, Wuhan Boster Biotechnology Company, Wuhan, Hubei Province, China) or rabbit anti-rat Nrf2 monoclonal antibody(1:150; PB0327, Wuhan Boster Biotechnology Company), as the primary antibody, and then with horseradish peroxidase-conjugated goat anti-rabbit IgG, as the secondary antibody (1:100; BA1055, Wuhan Boster Biotechnology Company).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Comparison

Journal: Neural Regeneration Research

Article Title: Electroacupuncture alleviates cerebral ischemia and reperfusion injury via modulation of the ERK1/2 signaling pathway

doi: 10.4103/1673-5374.187041

Figure Lengend Snippet: Effects of EA on GCS and Nrf2 immunoreactivities in the ischemic cortex. (A) Immunohistochemical staining for GCS and Nrf2 in the cortex in the different groups (× 400) ( n = 5 per group). (B, C) Mean optical densities of GCS and Nrf2-immunoreactive cells. Data are expressed as the mean ± SEM. Paired t -test was used to compare two groups, while analysis of variance followed by Tukey’s multiple comparisons test was used for multiple comparisons. ** P < 0.01, vs . sham (sham-operation) group; ## P < 0.01, vs . MCAO group; †† P < 0.01, vs . EA (MCAO + EA) group. EA: Electroacupuncture; GCS: glutamylcysteine synthetase; Nrf2: nuclear factor erythroid 2-related factor 2; MCAO: middle cerebral artery occlusion; EA plus PD98059: MCAO + EA + PD98059.

Article Snippet: Then, the sections were incubated with rabbit anti-rat GCS monoclonal antibody (1:100; BA1627, Wuhan Boster Biotechnology Company, Wuhan, Hubei Province, China) or rabbit anti-rat Nrf2 monoclonal antibody(1:150; PB0327, Wuhan Boster Biotechnology Company), as the primary antibody, and then with horseradish peroxidase-conjugated goat anti-rabbit IgG, as the secondary antibody (1:100; BA1055, Wuhan Boster Biotechnology Company).

Techniques: Immunohistochemical staining, Staining

Journal: PLOS ONE

Article Title: Chronic intermittent hypoxia aggravated diabetic cardiomyopathy through LKB1/AMPK/Nrf2 signaling pathway

doi: 10.1371/journal.pone.0296792

Figure Lengend Snippet: (A-E) Representative blot images and quantitative analysis of LKB1, AMPK, Nrf2, HO-1. (F-G) Representative blot images and quantitative analysis of Nuclear-Nrf2. (H-I) Representative images of Nuclear-Nrf2 and the quantitative analysis for Nuclear-Nrf2 (scale bar, 100 μm). n = 3. Data are presented as means ± S.E.M. * p < 0.05 and ** p < 0.01 vs. db/m group; Φ p < 0.05 and ΦΦ p < 0.01 vs. db/db group.

Article Snippet: Seal with 5% skim milk powder sealing solution for 2 h, adding primary antibody and incubating overnight at 4°C: Tubulin (1:10000, GTX628802) and Caspase-3(1:1000, #14220) from CST; p-AMPK (1:2000, AP0116) and HO-1(1:1000, A1346) from ABclonal; PI3K (1:1000, AF6241) from Affinity; LKB1 (1:1000, 10746-1-AP), p-LKB1 (1:5000, 80127-1-RR), AMPK (1:500, 10929-2-AP), p-AKT (1:5000, 66444-1-Ig), AKT (1:5000, 60203-2-Ig), GLUT4 (1:3000, 66846-1-Ig) from Proteintech; Bax (1:1000, GB12690) from Seville; Bcl-2 (1:1000, BA0412) and Nrf2 (1:1000, A0674) from BOSTER; Lamin-B1(1:1000, SI17-06) from Huabio.

Techniques:

Journal: PLOS ONE

Article Title: Chronic intermittent hypoxia aggravated diabetic cardiomyopathy through LKB1/AMPK/Nrf2 signaling pathway

doi: 10.1371/journal.pone.0296792

Figure Lengend Snippet: (A) The cell viability of H9C2 cells was treated with HG with 0, 10, 30, and 50mM for 24 and 48 h. (B) The H9C2 cells were treated with IH and HG for 24 and 48 h, and cell viability of H9C2 cells was measured. (C) The cell viability of H9C2 cells treated with IH and HG. n = 6. (D-E) Representative images of mitochondrial membrane potential and quantitative analysis of the JC-1 ratio. n = 4. (F-G) Representative images of ROS and quantitative analysis of ROS. (H-K) Representative blot images and quantitative analysis of LKB1, AMPK, and Nrf2. n = 3. Data are presented as means ± S.E.M. * p < 0.05 and ** p < 0.01 vs. CON group; Φ p < 0.05 and ΦΦ p < 0.01 vs. HG group.

Article Snippet: Seal with 5% skim milk powder sealing solution for 2 h, adding primary antibody and incubating overnight at 4°C: Tubulin (1:10000, GTX628802) and Caspase-3(1:1000, #14220) from CST; p-AMPK (1:2000, AP0116) and HO-1(1:1000, A1346) from ABclonal; PI3K (1:1000, AF6241) from Affinity; LKB1 (1:1000, 10746-1-AP), p-LKB1 (1:5000, 80127-1-RR), AMPK (1:500, 10929-2-AP), p-AKT (1:5000, 66444-1-Ig), AKT (1:5000, 60203-2-Ig), GLUT4 (1:3000, 66846-1-Ig) from Proteintech; Bax (1:1000, GB12690) from Seville; Bcl-2 (1:1000, BA0412) and Nrf2 (1:1000, A0674) from BOSTER; Lamin-B1(1:1000, SI17-06) from Huabio.

Techniques: Membrane

Journal: PLOS ONE

Article Title: Chronic intermittent hypoxia aggravated diabetic cardiomyopathy through LKB1/AMPK/Nrf2 signaling pathway

doi: 10.1371/journal.pone.0296792

Figure Lengend Snippet: (A-B) Representative images of mitochondrial membrane potential and quantitative analysis of the JC-1 ratio. (C-D) Representative images and quantitative analysis of ROS. (E-G) Representative blot images and quantitative analysis of AMPK and Nrf2. n = 3. Data are presented as means ± S.E.M. * p < 0.05 and ** p < 0.01 vs. CON group; Φ p < 0.05 and ΦΦ p < 0.01 vs. IH+HG group.

Article Snippet: Seal with 5% skim milk powder sealing solution for 2 h, adding primary antibody and incubating overnight at 4°C: Tubulin (1:10000, GTX628802) and Caspase-3(1:1000, #14220) from CST; p-AMPK (1:2000, AP0116) and HO-1(1:1000, A1346) from ABclonal; PI3K (1:1000, AF6241) from Affinity; LKB1 (1:1000, 10746-1-AP), p-LKB1 (1:5000, 80127-1-RR), AMPK (1:500, 10929-2-AP), p-AKT (1:5000, 66444-1-Ig), AKT (1:5000, 60203-2-Ig), GLUT4 (1:3000, 66846-1-Ig) from Proteintech; Bax (1:1000, GB12690) from Seville; Bcl-2 (1:1000, BA0412) and Nrf2 (1:1000, A0674) from BOSTER; Lamin-B1(1:1000, SI17-06) from Huabio.

Techniques: Membrane